DNA
Part:BBa_K4141001:Design
Designed by: Anna Anastasiou Group: iGEM22_Thessaloniki (2022-10-12)
miR-10b complementary sequence from Y-shaped hairpin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
During our design, we had to take into account the sequence of miR-21, the right orientation (reverse complement with miR-21), the illegal sequences from iGEM, the bulge number (number of unpaired bases for stability), and the rest of our parts' sequences, because they all have to act as a whole.
Source
This part was created with our algorithm for hairpin design.